Modified Agglutination Test for Pasteurella tularensis
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Modified agglutination test for Pasteurella tularensis.
Several modifications in technique were incorporated into the standard agglutination test for Pasteurella tularensis. Reciprocal shaking of all tubes in a Kahn shaker was introduced to increase the rate of agglutination and quantity of agglutinated cell mass, making it possible to report preliminary results within 4 hr. Increased incubation time at a higher temperature was used to favor the rat...
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Preparation of washed particles. Cell-free extracts of highly virulent P. tularensis strain Sm were prepared by sonic disintegration of cells as described by Rendina and AMills (1957). Twenty ml of undialyzed centrifuged (3000 x G for 30 min) sonic extract, containing 3.8 to 4.2 mg SN per ml, were centrifuged for 3 hr at 105,000 X G at 5 C. The supernatant was decanted and thc reddish precipita...
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6 Islam A. A new bone marrow biopsy needle with core securing device. J Clin Pathol 1982;35:359-64. 7 Ellman L. Bone marrow biopsy in the evaluation of lymphoma, carcinoma and granulomatous disorders. Am J Med 1976;60:1-6. 8 Burke JS. The value of the bone marrow biopsy in the diagnosis of hairy cell leukaemia. Am J Clin Pathol 1978;70:876-84. 9 Islam A, Catovsky D, Goldman JM, Galton DAG. Valu...
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Viable attenuated tularemia vaccines have been shown to bt. effective in immunizing mice and other animals against challenge with high].y virulent Pasteurella tularensis. Nonviable preparations, however, induced little or no resistance to challenge with even small numbers of virulent organisms. It seemed reasonable to expect that antigenicity would be retained to a greater extent if the organis...
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In an investigation (Sherstinsky, 1951; Yaniv, 1951) of the factors governing the virulence of Pasteurella tularensis (Bacterium tularense), the correlation between the activity of catalase and virulence was studied. This line of approach was suggested by the fact that in the Brucella group (Huddleson and Stahl, 1943) and in PasteureUa pestis (Rockenmacher, 1949) high virulence appears to be as...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1966
ISSN: 0003-6919
DOI: 10.1128/aem.14.2.267-270.1966